Laboratory of Molecular Biology

 

Head: Prof. Andrzej Lewandowski

Scientific staff:

  • Prof. Andrzej Lewandowski Adres poczty elektronicznej jest chroniony przed robotami spamującymi. W przeglądarce musi być włączona obsługa JavaScript, żeby go zobaczyć.
  • Dr. Witold Wachowiak Adres poczty elektronicznej jest chroniony przed robotami spamującymi. W przeglądarce musi być włączona obsługa JavaScript, żeby go zobaczyć.

Technical staff:

  • Monika Litkowiec M.Sc. Adres poczty elektronicznej jest chroniony przed robotami spamującymi. W przeglądarce musi być włączona obsługa JavaScript, żeby go zobaczyć.
  • Maria Ratajczak


Research interests

Laboratory of Molecular Biology was created on the 1st of September 2003. We are interested in applying molecular markers to study spatial and genetic structures of population, natural hybridization, adaptation, molecular phylogenetics of native tree species. We also use allozymes markers to describe genetic variation of population and study trees' mating system.


List of selected grants and scientific studies:

  • Geographic structure of Norway Spruce mtDNA polymorphism in Poland.
    The project is financed by The Ministry of Science and Higher Education.
  • Development of molecular based methods for identification and early evaluation of reforestation material and implementation of the methods to the forestry.
    The project is financed by The National Forests and worked in cooperation with the Department of Genetics, University of Bydgoszcz and Forest Research Institute.
  • Reproductive processes on Norway Spruce second generation seeds plantation.
    The project is financed by the Ministry of Science and Higher Education and worked in cooperation with The Laboratory of Genetics ID PAS.
  • Genetic and geographic differentiation of Juniperus oxycedrus L. and related taxa of Juniperus genus (Cupressaceae) from Pleistocene refugia.
    The project is financed by The Ministry of Science and Higher Education and worked in cooperation with the Laboratory of Systematic and Geography ID PAS.
  • Adaptation, speciation and phylogeography of the taxa from the Pinus mugo complex in Europe.
    Grant from the Ministry of Science and Higher Education, Poland.
  • Genomics of adaptive variation and demography in Scots pine (Pinus sylvestris L.).
    Grant from the Ministry of Science and Higher Education, Poland.


Selected publications:

  • Wachowiak W., Palmé A.e., Savolainen O. 2011. Speciation history of three closely related pines Pinus mugo (T.), P. uliginosa (N.) and P. sylvestris (L.). Molecular Ecology, DOI: 10.1111/j.1365-294X.2011.05037.x
  • Salmela, M. J., Cavers, S., Wachowiak, W., Cottrell, J. E., Iason, G. R. & Ennos, R. A. 2010. Understanding the evolution of native pinewoods in Scotland will benefit their future management and conservation. Forestry, doi:10.1093/forestry/cpq036
  • Wachowiak W., Salmela M.J., Ennos R.E., Iason G., Cavers S. 2010. High genetic diversity at the extreme range edge: nucleotide variation at nuclear loci in Scots pine (Pinus sylvestris L.) in Scotland. Heredity, doi: 10.1038/hdy.2010.118
  • Jasińska A.K., Wachowiak W., Muchewicz E., Boratyńska K., Montserrat J.M., Boratyński A. 2010. Cryptic hybrids between Pinus uncinata and P. sylvestris. Botanical Journal of the Linnean Society, 163: 473-485.
  • Trocha L.K., Mucha J., Eissenstat D.M., Reich P.B., Oleksyn J. Ectomycorrhizal identity determines respiration and concentrations of nitrogen and non-structural carbohydrates in root tips: a test using Pinus sylvestris and Quercus robur saplings. Tree Physiology (in press, doi: 10.1093/treephys/tpq014)
  • Dering M., Lewandowski A. 2009. Finding the meeting zone: Where have the northern and southern ranges of Norway spruce overlapped? Forest Ecology and Management 259: 229-235.
  • Litkowiec M., Dering M., Lewandowski A. 2009. Utility of two mitochondrial markers for identification of Picea abies refugial origin. Dendrobiology 61: 65-71.
  • Wachowiak W., Prus-Glowacki W. 2009. Different patterns of genetic structure of relict and isolated populations of endangered peat-bog pine (Pinus uliginosa Neumann). Journal of Applied Genetics 50(4), 329-339.
  • Wachowiak W., Balk P.A., Savolainen O. 2009. Search for nucleotide diversity patterns of local adaptation in dehydrins and other cold-related candidate genes in Scots pine (P. sylvestris L.). Tree Genetics & Genomes 5: 117-132
  • Palmé A.E, Pyhäjärvi T., Wachowiak W., Savolainen O. 2009. Selection on Nuclear Genes in a Pinus Phylogeny. Molecular Biology and Evolution 26: 893 - 905.
  • Chałupka W., Mejnartowicz L, Lewandowski A. 2008. Reconstruction of a lost forest tree population: A case study of Nowary spruce (Picea abies [L.] Karst.). Forest Ecology and Management 255: 2103-2108.
  • Dering M., Lewandowski A., Ufnalski K., Kędzierska A. (2008). How far to the east was the migration of white oaks from the Iberia refugium? Silva Fennica 42(3): 327-335.
  • Wachowiak W., Prus-Głowacki W. (2008). Hybridization processes in sympatric populations of pines Pinus sylvestris L., P. mugo Turra and P. uliginosa Neumann. Plant Systematics and Evolution DOI 10.1007/s00606-007-0609-z.
  • Dering M., Lewandowski A. (2007). Unexpected disproportion observed in species composition between oak mixed stands and their progeny populations. Annals of Forest Science 64: 413-418.
  • Kosinska J., Lewandowski A., Chalupka W. (2007). Genetic Variability of Scots Pine Maternal Populations and Their Progenies. Silva Fennica 41: 5-12.
  • Wachowiak W., Bączkiewicz A., Chudzińska E., Buczkowska K. (2007). Cryptic speciation in bryophytes - a case study in the Aneura pinguisBotanical Journal of the Linnean Society 155, 273-282. complex.
  • Wachowiak W., Odrzykoski I., Myczko Ł., Prus-Głowacki W. (2006). Lack of evidence on hybrid swarm in the sympatric population of Pinus mugo and P. sylvestris. Flora 4: 307-316.
  • Wachowiak W., Stephan B.R., Schulze I., Prus-Głowacki W., Ziegenhagen B. (2006). A critical evaluation of reproductive barriers between closely related species using DNA markers - a case study in Pinus. Plant Systematics and Evolution 257: 1-8.
  • Lewandowski A., Dering M. (2006). Crossability between Pinus uliginosa and its putative parental species Pinus sylvestris and Pinus mugo. Silvae Genetica 55: 52-54.
  • Buczkowska K., Adamczak M., Chudzińska E., Wachowiak W., Bączkiewicz A. (2006). In vitro propagation of cryptic species of Aneura pinguisCryptogamie Briologie 27(2), 241-251. (Hepaticae, Metzgeriales).
  • Lewandowski A., Burczyk J., Wachowiak W., Boratyński A., Prus-Głowacki W. (2005). Genetic evaluation of seeds of highly endangered Pinus uliginosa Neumann from Wegliniec reserve for ex-situ conservation program. Acta Societatis Botanicorum Poloniae 74 (3): 237-242.
  • Bączkiewicz A., Buczkowska K., Wachowiak W. (2005). Anatomical and morphological variability of needles of Pinus mugo Turra on different substrata in the Tatra Mountains. Biological Letters 42(1): 21-32.
  • Wachowiak W., Celiński K., Prus-Głowacki W. (2005). Evidence of natural reciprocal hybridisation between Pinus uliginosa and P. sylvestris in the sympatric population of the species. Flora 200, 563-568.
  • Adamczak M., Buczkowska K., Baczkiewicz A., Wachowiak W. (2005). Comparison of allozyme variability in Polish populations of two species of Ptilidium Nees (Hepaticae) with contrasting degrees of sexual reproduction. Cryptogamie Bryologie 26 (2): 151-165.
  • Wachowiak W., Lewandowski A., Prus-Głowacki W. (2005). Reciprocal controlled crosses between Pinus sylvestrisP. mugo verified by a species-specific cpDNA marker. Journal of Applied Genetics 46 (1) 41 - 43. and
  • Wachowiak W., Bączkiewicz A., Celiński K., Prus-Głowacki W. (2004). Species-specific chloroplast DNA polymorphism in the trnV-rbcL region in Pinus sylvestris and P. mugo. Dendrobiology 51: 67-72.
  • Boratyński A., Boratyńska K., Lewandowski A., Gołąb Z., Kiciński P. (2003). Evidence of the possibility of natural reciprocal crosses between Pinus sylvestris and P. uliginosa based on the phenology of reproductive organs. Flora 198: 377-388.