Laboratory of Vegetative Reproduction

 

Head:Prof. Krystyna Bojarczuk

Scientific staff:

  • Prof. Krystyna Bojarczuk Adres poczty elektronicznej jest chroniony przed robotami spamującymi. W przeglądarce musi być włączona obsługa JavaScript, żeby go zobaczyć.

  • Dr Teresa Hazubska-Przybył Adres poczty elektronicznej jest chroniony przed robotami spamującymi. W przeglądarce musi być włączona obsługa JavaScript, żeby go zobaczyć.

Technical staff:

  • Elżbieta Sobczak


Research interests

Research on vegetative propagation has a long tradition and has always been closely associated with collections of trees, shrubs and other plants, where studies of effects of climatic and soil conditions on plant growth and flowering are carried out. We have developed and put into practice new methods of propagation (from cuttings and by grafting) of important ornamental shrubs: lilacs, magnolias, azaleas, and rhododendrons. At present we conduct experiments on propagation of various trees and shrubs by means of tissue culture (so-called micropropagation). This method has given very good results for lilacs, rhododendrons, azaleas, and selected clones of polar and birch. We conduct research on somatic embryogenesis of selected coniferous trees species of the genera: Picea, Pinus and Abies. Beside micropropagation, we pursue experiments on the influence of some stress factors (e.g. toxic ions, high or low pH of the medium) on the development of roots and shoots of selected trees and shrubs. The experiments aim at selecting plants tolerant to unfavourable environmental conditions. Investigations are financed by Institute of Dendrology and Committee for Scientific Investigations.

Collaborative research

The Laboratory cooperates with various scientific institutions in Poland and abroad, including the Research Institute for Nursery Stock, Boskoop, Holland, and INRA Orleans Research Centre, Forestry Institute, France).

List of selected grants and scientific studies

List of selected grands and studies carried out (since 1997) by the Laboratory of Vegetative Reproduction staff members:

  • Somatic embryogenesis and cryopreservation of embryogenic cultures of Picea abies (L.) Karst.and P. omorika (Pancić) Purk. by the vitrification method (MNiSW grant 2009-2012)

  • Influence of ectomycorrhizal inoculation on the acclimatization of poplar and birch microplants cultured in vitro in metal-contaminated soil (MNiSW grant 2008-2011).

  • Application of chemical mutagens and laser beams to obtain rhododendrons with increased tolerance to disadvantageous soil conditions (project financed by the Committee for Scientific Research).

  • Somatic embryogenesis of some spruce: Picea abies, P. omorika, P. pungens 'Glauca', P. breweriana. (project financed by the Committee for Scientific Research).

  • Organogenesis and regeneration of some trees and shrubs in vitro (project financed by the Institute of Dendrology).

  • Development of birch (Betula pendula L.) and gray poplar (Populus tremula L. x P. alba L.) cultured in vitro and ex vitro on polluted media (project financed by the Committee for Scientific Research).

  • Initiation of embryogenic cultures and development of somatic embryos of selected ornamental spruce species (project financed by the Committee for Scientific Research).

  • Development of poplar and birch in vitro and ex vitro under the influence of stress caused by toxic metal ions (project financed by the Committee for Scientific Research).

Selected publications

  • Bojarczuk K., Kieliszewska-Rokicka B. 2009. Effect of Ectomycorrhiza on Cu and Pb Accumulation in Leaves and Roots of Silver Birch (Betula pendula Roth.) Seedlings Grown in Metal-Contaminated Soil. Water, Air and Soil Pollution DOI 10.1007 sl 1270-009-0131

  • Hazubska-Przybył T., Bojarczuk K. 2008. Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana). Acta Societatis Botanicorum Poloniae 77, 3: 189-199

  • Hazubska-Przybył T., Bojarczuk K., Guzicka M. 2008 Structure of embryogenic tissues and accumulation of storage materials in somatic embryos of Picea abies and P. omorika. Dendrobiology 60: 19-28.

  • Szczygieł K., Hazubska-Przybył T., Bojarczuk K. 2007. Somatic embryogenesis of selected coniferous tree species of the genera Picea, AbiesLarix. Acta Soc. Bot. Pol. 76,1: 7-15. and

  • Bojarczuk K., Oleksyn J., Karolewski P., Żytkowiak R. 2006. Response of silver birch (Betula pendula Roth.) seedlings to experimental variation in aluminium concentration. Polish Journal of Ecology 53, 2: 189-199.

  • Bojarczuk K., Przybył K. 2005. Effect of polluted substances on growth and health of silver birch (Betula pendula Roth.). Polish Journal of Env. Stud. 14, 5: 677-684.

  • Bojarczuk K., Szczygieł K. 2004. Effect of aluminium and copper on the development of birch (Betula pendula Roth.) cultured in vitro and in vivo. Dendrobiology 51: 3 -8.

  • Bojarczuk K. 2004. Effect of Toxic Metals on the Development of Poplar (Populus tremula L. x P. alba L.) Cultured in vitro. Polish Journal of Environmental Studies 13, 2: 115-120.

  • Bojarczuk K. 2004. Effect of Aluminium on the Development of Poplar (Populus tremula L. x P. alba L.) in vitro and in vivo. Polish Journal of Environmental Studies 13, 3: 261-266.

  • Giel P., Bojarczuk K. 2004. Einfluss hoher Ca2+ - und HCO3- Konzentrationen auf die Entwicklung der Microstecklinge des Rhododendrons 'Catawbiense Grandiflorum' in der in vitro Kulturen. Rhododendron und immergrüne Laubgehölze Jahrbuch 2004: 29-41.

  • Hazubska T., Szczygieł K. 2003. Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens 'Glauca', P. breweriana and P. abies. Dendrobiology 50:17-24.

  • Bojarczuk K. 2002. Effect of toxic metal ions on the development of poplar (Populus tremula L. x Populus alba L.) cultured in vitro. Zeszyty Problemowe Postepów Nauk Rolniczych 488:535-544.

  • Bojarczuk K., Karolewski P., Oleksyn J., Kieliszewska-Rokicka B., Żytkowiak R., Tjoelker M.G. 2002. Effect of Polluted Soil and Fertilisation on Growth and Physiology of Silver Birch (Betula pendula Roth.) Seedlings. Polish Journal of Environmental Studies vol. 11, 5:483-492.

  • Bojarczuk K., Oleksyn J., Karolewski P., Żytkowiak R. 2002. Effect of aluminium compounds on seedling development in silver birch (Betula pendula Roth.). Conf. Proc. pp: 685-692.

  • Giel P., Bojarczuk K. 2002. The effect of high concentration of selected calcium salts on development of microcuttings of rhododendron R. 'Catawbiense Grandiflorum' in in vitro cultures. Dendrobiology vol. 48:23-29.

  • Giel P., Bojarczuk K. 2001. The influence of high level of calcium and pH in the substrate on Rhododendrons. Erica Polonica 12: 95-101.

  • Bojarczuk K. 2000. Effect of aluminium on in vitro rooting of birch (Betula pendula Roth.) and poplar (Populus tremula L. x P. alba L.) microcuttings. Acta Societatis Botanicorum Poloniae v. 69, 4: 251-255.

  • Bojarczuk K. 2000. Effect of calcium and various pH levels in the media on regeneration of Rhododendron in vitro culture. Zeszyty Problemowe Postępów Nauk Rolniczych v. 473: 31-38.

  • Bojarczuk K. 1999. Effect of aluminium toxicity on the development of poplar (Populus tremula L. x P. alba L.) cultured in vitro. Acta Soc. Bot. Poloniae vol. 68, 4: 245-250.

  • Bojarczuk K., Bojarczuk T. 1999. Vegetative propagation of maples. In: Maples - Acer camperstre L., Acer platanoides L. Ed. W. Bugała. Publ. Bogucki, Poznań: 229- 256.

  • Bojarczuk K. 1998. Influence of aluminium toxicity on the development of poplar (Populus tremula L. x P. alba L.) cultured in vitro. Acta Physiol. Plantarum 20: 5-6

  • Bojarczuk K. 1997. Influence of aluminium toxicity on the development of birch (Betula pendula Roth.) microcuttings cultured in vitro. Acta Physiol Plantarum 19: 199.

  • Bojarczuk K. 1997. Influence of aluminium on the development of birch (Betula pendula Roth.) microcuttings cultured in vitro. Arbor. Kórnickie 42: 217-228.

  • Bojarczuk K. Kieliszewska-Rokicka B. 1997. Physiological properties on some Rhododendron species differing in rootability of cuttings. Erica: 25-34

  • Bojarczuk K. 1996. Regeneration of several Rhododendron cultivars from axillary and adventitious buds from in vitro cultures. Arbor. Kórnickie 41: 109-126.

  • Bojarczuk K. 1996. Multiplication of chosen varieties of Rhododendron from shoot-seedlings and tissue cultures. Cof. Proc. Zjazd Sekcji Dendrologicznej: 131-146.

  • Bojarczuk K. 1995. Regeneration of selected species and cultivars of Rhododendron from cuttings and from organ cultures in vitro. Plantpress. Kraków:1-112.

  • Bojarczuk K., Oleksyn J. 1995 Development of pine (Pinus sylvestris L.) and birch (Betula pendula Roth) seedlings on polluted substrates inoculated with the fungus Trichoderma harzianum Rifai. Arbor. Kórnickie 39: 163-177.

  • Bojarczuk K. 1995. In vitro and ex vitro rooting of microcuttings some Rhododendron cultivars. Erica, No. 6. Erica: 29-37