INSTYTUT DENDROLOGII

Head: Dr. Ewelina Ratajczak- Ten adres pocztowy jest chroniony przed spamowaniem. Aby go zobaczyć, konieczne jest włączenie w przeglądarce obsługi JavaScript.

Staff:

Dr. Ewa Kalemba - Ten adres pocztowy jest chroniony przed spamowaniem. Aby go zobaczyć, konieczne jest włączenie w przeglądarce obsługi JavaScript.

Shirin Alipour M.Sc. - Ten adres pocztowy jest chroniony przed spamowaniem. Aby go zobaczyć, konieczne jest włączenie w przeglądarce obsługi JavaScript.

Joanna Kijowska-Oberc M.Sc. - Ten adres pocztowy jest chroniony przed spamowaniem. Aby go zobaczyć, konieczne jest włączenie w przeglądarce obsługi JavaScript.

Anna Kołacz M.Sc. - Ten adres pocztowy jest chroniony przed spamowaniem. Aby go zobaczyć, konieczne jest włączenie w przeglądarce obsługi JavaScript.

Natalia Wojciechowska M.Sc. - Ten adres pocztowy jest chroniony przed spamowaniem. Aby go zobaczyć, konieczne jest włączenie w przeglądarce obsługi JavaScript.

Danuta Ratajczak

Research interests:

• The mechanisms of desiccation tolerance of tree seeds (Acer platanoides, Acer pseudoplatanus, Acer saccharinum, Fagus sylvatica, Quercus robur)
  1. Acquisition and loss of desiccation tolerance
  2. Role of oligosaccharides in desiccation tolerance
     1. oligosaccharide accumulation
     2. oligosaccharide metabolism
  3. Role of ABA in desiccation tolerance
  4. Oxidative damages and defense reactions in orthodox and recalcitrant seeds during desiccation. The role of ascorbate-glutathione pathways.
  5. Role of LEA and sHSP proteins in desiccation tolerance
  6. Role of thiol compounds and redox state in desiccation tolerance.
  7. The improvement of the tolerance to desiccation of recalcitrant seeds.
• Biochemical basis of longevity of tree seeds
  1. Sensibility of beech (Fagus sylvatica L.) seeds to changed temperature and moisture conditions during storage
     1. viability
     2. oxidative stress and defense reactions
  2. Sensibility of beech seeds to drying conditions effect of temperature drying on viability and seed storability
  3. Water relations in beech seeds
     1. free water content in embryo axes and cotyledons
     2. water sorption by embryo axes and cotyledons (water sorption isotherms)
     3. glass transition in beech seeds studied by spin label electron paramagnetic resonance
  4. The role of LEA and sHSP proteins in seed longevity
  5. The role of peroxiredoxins and redox state in seed longevity
  6. Protein carbonylation of Fagus sylvatica seeds during long-term storage

List of selected grants and scientific research

• Adaptation of Scots pine to environmental pollution. 1996-1999 (P.M. Pukacki, S. Pukacka, A. Górska).
• Biochemical basis of reproductivity of Fagus sylcatica seeds. 2000-2003, (S. Pukacka, E. Ratajczak, P.M. Pukacki)
• Molecular and physiological factors influenced longevity of beech (Fagus sylvatica L) seeds. 2004 - 2007. (S. Pukacka, E. Ratajczak, E. Kalemba)
• Functional analysis of dehydrins and LEA-like proteins in seeds of broadleaves species. 2008-2011, (E.M. Kalemba, E. Ratajczak, S. Pukacka)
• The role of peroxiredoxins and changes in redox state of seed cells of broadleaves species during development desiccation and storage. 2009 - 2012. (E. Ratajczak, E.M. Kalemba, S. Pukacka).
• Protein carbonylation in beech (Fagus sylvatica L.) seeds during long term storage. 2010-2011 (E. Kalemba)
• Somatic embryogenesis and cryoconservation of embryogenic cultures of Picea abies (L.) Karst. and Picea omorika (Pancić) Purk. by the vitrification method. 2009-2012 (E. Ratajczak, E. Kalemba - performers)
• Seeds as genetic sources of black poplar (Populus nigra L.), threatened species in Poland. 2010-2013 (E. Ratajczak, E. Kalemba - performers)
• The regulatory effect of glucose and nitrate on the accumulation and degradation of storage lipids in seeds of yellow lupine (Lupinus luteus L.), white lupine (Lupinus albus L.) and andean lupine (Lupinus mutabilis Sweet) 2011-2014 (S. Pukacka - performer)
• Secondary sexual dimorphism in dioecious plants. Evolutionary adaptation or a consequence of different resource allocation strategies? 2013-2016  (E. Ratajczak– performer)
• The involvement of proteins containing an oxidized methionine and a methionine sulfoxide reductase system in the regulation of important developmental stages and physiology in seeds. 2016-2020 (EM Kalemba – principal investigator)

Selected publications:

• Kalemba EM, Stolarska E 2019. Regulation of gene expression of methionine sulfoxide reductases and their new putative roles in plants. Int J Mol Sci. 20(6). pii: E1309.
• Ratajczak E, Dietz KJ, Kalemba EM 2019 The occurrence of peroxiredoxins and changes in redox state in Acer platanoides and Acer pseudoplatanus during seed development. Journal Plant Growth 38Ł 298-314.
• Ratajczak E, Staszak AM, Wojciechowska N, Bagniewska-Zadworna A, Dietz KJ 2019 Regulation of thiol metabolism as a factor that influences the development and storage capacity of beech seeds. Journal of Plant Physiology 239:61-70.
• Kurek K,  Plitta-Michalak B, Ratajczak E 2019 Reactive oxygen species as potential drivers of the seed aging process. Plants (Basel) 14;8(6) pii: E174.
• Ratajczak E, Malecka A, Ciereszko I, Staszak AM 2019 Mitochondria are important determinants of the aging of seeds. International Journal of Molecular Sciences 20(7): 1568.
• Małecka A, Konkolewska A, Hanc A, Barałkiewicz D, Ciszewska L, Ratajczak E, Staszak AM, Kmita H, Jarmuszkiewicz W Insight into phytoremediation capability of Brassica juncea (v. Malopolska): metal accumulation and antioxidant enzymes activity. IJMS Molecular Plant Sciences  5;20(18). pii: E4355.
• Kalemba EM, Ratajczak E 2018 The effect of a doubled glutathione level on parameters affecting the germinability of recalcitrant Acer saccharinum seeds during drying. Journal of Plant Physiology 223:72-83
• Robakowski P, Pers-Kamczyc E, Ratajczak E, Thomas PA, Ye ZP, Rabska M, Iszkuło G 2018 Photochemistry and Antioxidative Capacity of Female and Male Taxus baccata L. Acclimated to Different Nutritional Environments Frontiers Plant Science 9:742
• Bogdziewicz M, Bonal R, Espelta JM, Kalemba EM, Steele MA, Zwolak R. 2018 Invasive oaks escape pre-dispersal insect seed predation and trap enemies in their seeds. Integr Zool 13(3):228-237
• Wojciechowska N, Marzec-Schmidt K, Kalemba EM, Zarzyńska-Nowak A, Jagodziński AM, Bagniewska-Zadworna A. Autophagy counteracts instantaneous cell death during seasonal senescence of the fine roots and leaves in Populus trichocarpa. BMC Plant Biol. 2018, 18(1):260.
• Ratajczak E, Dietz KJ 2017 Regulatory redox state in tree seeds. Acta Societatis Botanicorum Poloniae 86(4):35-67
• Borek S, Kalemba EM, Pukacka S, Pietrowska-Borek M, Stawiński S, Ratajczak L 2017 Nitrate simultaneously enhances lipid and protein accumulation in developing yellow lupin cotyledons cultured in vitro, but not under field conditions. Journal Plant Physiol. 216:26-34.
• Hazubska- Przybył T.,Kalemba E.M., Ratajczak E., Bojarczuk K. 2016. Effects of abscisic acid and an osmoticum on the maturation,starch accumulation and germination of Picea spp. Somatic embryos. Acta Physiol. Plant. 38:59
• Kalemba E.M., Litkowiec M. 2015. Functional characterization of a dehydrin protein from Fagus sylvatica seeds using experimental and in silico approaches. Plant Physiol Bioch. 97:246-54
• Kalemba E.M., Bagniewska-Zadworna A., Ratajczak E. 2015. Multiple subcellular localizations of dehydrin-like proteins in the embryonic axes of common beech (Fagus sylvatica L.) seeds during maturation and dry storage. J.Plant Growth Regul. 34:137-149
• Kalemba E.M., Suszka J., Ratajczak E. 2015.The role of oxidative stress in determining the level of viability of black poplar (Populusnigra) seeds stored at different temperatures. Funct Plant Biol 42:630-642
• Ratajczak E., Małecka A., Bagniewska-Zadworna A., Kalemba E.M.2015.The production, localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech (Fagus sylvatica L.) seeds. J Plant Physiol. 174:147–156
• Ratajczak E, Kalemba E.M., Pukacka S.2015. Age-related changes in protein metabolism of beech (Fagus sylvatica L.) seeds during alleviation of dormancy and in early stage of germination. Plant Physiol.Bioch. 94:114-121
• Kalemba E.M., Pukacka S. 2014. Carbonylated proteins accumulated as vitality decreases during long-term storage of beech (Fagus sylvatica L.) seeds. Trees 28: 503-515
• Mucha J., Guzicka M., Ratajczak E., Zadworny M. 2014. Strategies utilized by trophically diverse fungal species for Pinus sylvestris root colonization. Tree Physiol. 34:73-86
• Pukacka S., Ratajczak E. 2014. Factors influencing the storability of Fagus sylvatica L. seeds after release from dormancy. Plant Growth Reg 72:17-27
• Hazubska-Przybyl T., Ratajczak E., Kalemba E.M., Bojarczuk K. 2013. Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea species. Dendrobiology 69: 77-86
• Pukacka S., Ratajczak E. 2013. Factors influencing the storability of Fagus sylvatica L. seeds after release from dormancy. Plant Growth Reg 72:17-27
• Ratajczak E., Ströher E., Oelze M.L., Kalemba E.M., Pukacka S., Dietz K.J . 2013. The involvement of the mitochondrial peroxiredoxin Prx IIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer species. Funct. Plant Biol 40:1005-1017
• Kalemba E.M., Pukacka S. 2012.Association of protective proteins with dehydration and desiccation of orthodox and recalcitrant category seeds of three Acer genus species. J Plant Growth Regul 31:351-362.
• Kalemba E.M., Pukacka S. 2012.Association of protective proteins with dehydration and desiccation of orthodox and recalcitrant category seeds of three Acer genus species. J.Plant Growth Regul 31;351-362.
• Borek S.,Pukacka S., Michalski K. 2012. Regulation by sucrose of storage compounds breakdown in germinating seeds of yellow lupine (Lupinus luteusL.), white lupine (Lupinus albusL.) and Andean lupine (Lupinus mutabilis Sweet). II. Mobilization of storage lipid. Acta Phys. Plant 34:1199-1206.
• Pukacka S., Malec M., Ratajczak E. 2011. ROS production and antioxidative system activity in embryonic axes of Quercus robur seeds under different desiccation rate conditions. Acta Physiol Plant 33: 2219-2227.
• Pukacka S., Ratajczak E., Kalemba E. 2011. The protective role of selenium in recalcitrant Acer saccharinum L. seeds subjected to desiccation. J Plant Physiol. 168: 220-225.
• Pukacka S., Ratajczak E. 2010. Ascorbate and glutathione metabolism during development and desiccation of beech (Fagus sylvatica L.) seeds. Plant Growth Regul. 62: 77-83.
• Pukacka S., Ratajczak E., Kalemba E. 2009. Non-reducing sugar levels in beech (Fagus sylvatica) seeds as related to withstanding desiccation and storage. J Plant Physiol. 166: 1381-1390.
• Borek S., Pukacka S., Michalski K., Ratajczak L. 2009. Lipid and protein accumulation in developing seeds of three lupine species: Lupinus luteus L., Lupinus albus L. and Lupinus mutabilis Sweet. J Exp Bot. 60: 3453-3466
• Kalemba E., Janowiak F, Pukacka S. 2009. Desiccation tolerance acquisition in developing beech (Fagus sylvatica L.) seeds: the contribution of dehydrin-like protein. Trees 23: 305-315.
• Kalemba E.M., Pukacka S. 2008. Changes in late embryogenesis abundant proteins and small heat shock protein during storage of beech (Fagus sylvatica L.) seeds. Env Exp Bot 63:274-280.
• Pukacka S. Ratajczak E. 2007. Age-related biochemical changes during storage of beech (Fagus sylvatica L.) seeds. Seed Sci Res 17: 45-53.
• Pukacka S., Ratajczak E. 2007. Ascorbate and glutathione metabolism during development and desiccation of orthodox and recalcitrant seeds of the genus Acer. Funct.Plant Biol 34: 601-613.
• Kalemba E., Pukacka S. 2007. Possible roles of LEA proteins and sHSPs in seed protection , a short review. Biological Lett 44:1-8
• Pukacka S. 2007. Hormonal regulation of growth and development. In: M.G.Tjoelker, A. Boratyński, W. Bugała (eds). Biology and Ecology of Norway Spruce pp: 71- 80. Springer
• Ratajczak E., Pukacka S. 2006. Changes in the ascorbate-glutathione system during storage of recalcitrant seeds of Acer saccharinum L. Acta Soc Bot Pol 1: 23-27.
• Pukacka S. Ratajczak E. 2006. Antioxidative response of ascorbate-glutathione pathway enzymes and metabolites to desiccation of recalcitrant Acer saccharinum seeds. J Plant Physiol 163:1259-1266
• Pukacka S., Ratajczak E. 2005. Production and scavenging of reactive oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity. J Plant Physiol 162:873-885
• Ratajczak E., Pukacka S. 2005. Decrease of beech (Fagus sylvatica) seed viability caused by temperature and humidity conditions as related to membrane damage and lipid composition. Acta Physiol Plant 27.1.3-12.
• Pukacka S., Wójkiewicz E. 2003. The effect of the temperature of drying on viability and some factors affecting storability of Fagus sylvatica. Acta Physiol Plant 25:163-169. seeds.
• Pukacka S., Hoffmann S.K., Goslar J., Pukacki P.M.,Wójkiewicz E. 2003. Water and lipid reactions in beech (Fagus sylvatica L.) seeds and its effect on storage behaviour. Bioch Biophys. Acta 1621: 48-56.
• Pukacka S., Wójkiewicz E. 2002. Carbohydrate metabolism in Norway maple and sycamore seeds in relation to desiccation tolerance. J Plant Physiol 159: 273-279.