- Written by: Agnieszka Szuba, Anita Rzadkiewicz
We kindly invite you to listen to the workshop led by Dr. Agnieszka Szuba and M.Sc. Anita Rzadkiewicz, titled “Proteins from the inside – how are proteomic studies conducted?”
We isolate proteins from plant material, such as leaves or tree roots, yielding extracts that can contain mixtures of up to thousands of proteins. These mixtures are then separated using electrophoretic methods. Proteins resolved in gels are either stained or transferred onto specialized membranes, which are then used for immunodetection. By analyzing the resulting protein images, we select bands or spots containing the proteins of interest and subsequently identify them using mass spectrometry methods.
We kindly invite you to listen to the workshop led by Prof. Paweł Chmielarz, Dr. Mikołaj K. Wawrzyniak and Dr. Jan Suszka, titled “A kitchen full of seeds and agar.”
Seeds have developed a range of diverse traits that regulate their germination. During the workshop, we will present research methods and tests that enable proper storage of woody species seeds under controlled conditions and allow for a closer examination of the seed germination process and seedling growth. We will also demonstrate how to break seeds out of a deep physiological dormancy. Furthermore, we will introduce modern approaches to preserving genetic resources in the form of seeds. The results of our research are applied in forestry practice and in gene banks.
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- Written by: Ewa Kalemba
We kindly invite you to listen to the workshop led by Prof. Ewa M. Kalemba, titled “Visualization and measurement of reactive oxygen species in germinating seeds.”
Reactive oxygen species (ROS) serve a signaling function during seed germination and enable the development of normal seedlings. They are produced in the apical meristem of the embryonic root. For visualization, a specific substrate is used that is converted into a precipitate visible to the naked eye. A brown color indicates the site of hydrogen peroxide (H2O2) synthesis, whereas black indicates the superoxide anion (O2•–). Subsequently, their quantities are precisely measured.
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- Written by: E. Ratajczak, H. Fuchs, J. Kijowska-Oberc
We kindly invite you to listen to the workshop led by Prof. Ewelina Ratajczak, Dr. Hanna Fuchs and M.Sc. Joanna Kijowska-Oberc, titled “Biodiversity measured by breath.”
Tree seeds differ not only in shape and size but also in biochemical traits, such as respiration rate. This rate is influenced by the climatic conditions in which the seeds mature. High temperatures and low precipitation accelerate the aging process of seeds, reducing their respiration rate. Consequently, the germination process—crucial for preserving forest biodiversity—becomes disrupted. By using the Agilent Seahorse XFp device, it is possible to monitor respiration in seed cells to identify those most resistant to environmental stress caused by global warming.
