Danuta Kulpa, Marcin Kubus
Micropropagation of Calycanthus fertilis
Dendrobiology 2010, vol. 64: 31-35
Abstract: Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on method propagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg・dm-3) and TDZ (from 0.1 to 0.5 mg・dm-3). BAP turned out to significantly increase initiation frequency whereas TDZ inhibited the formation of adventitious shoots and caused explant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg・dm-3 BAP. Primary explants which initiated growth were transferred on the proliferation media containing WPM macro and microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg・dm-3), KIN (1.0 to 5.0 mg・dm-3) or TDZ (from 0.1 to 0.5 mg・dm-3). Calycanthus multiplication in vitro should be conducted on WPM media with 1.0 mg・dm-3 BAP. Proliferated shoots were placed on the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg・dm-3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg・dm-3 IBA. To sum up, it should be stated that an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.
Additional key words: in vitro cultures, multiplication, plant growth regulators