Sudipta Shekhar Das Bhowmik, Tsering Stobdan, Lingaraj Sahoo
Germination and short-term storage of Hippophae rhamnoides L. seeds and its ex-situ reintroduction potential assessment under North East Indian conditions
Dendrobiology 2013, vol. 70: 03-12
http://dx.doi.org/10.12657/denbio.070.001
Abstract: Germination efficiency of freshly harvested Hippophae rhamnoides seeds collected from cold desert of Ladakh outside its native place under in vivo glass house condition recorded a highest of 73% germination in soilrite at 22±2°C and 60–70% RH within three weeks at Guwahati, North East India. Germination of seeds was significantly enhanced to a maximum of 90% under in vitro condition in 1/4th Murashige & Skoog (MS) medium supplemented with 3% sucrose and 2% activated charcoal within two weeks. Glass house acclimatized healthy seedlings after been introduced to natural climatic conditions of Shillong and Guwahati of North East India showed inadequate survivability. Unlike storage at room temperature which is detrimental for seed viability, low temperature storage under refrigeration and natural climatic condition of Ladakh retains viability for prolonged period.
Additional key words: in vitro, in vivo, polyphenols, viability, introduction, substratumgermination rate, pollen morphology, polyploidy