Research focus:
In my scientific research I investigate the issue of longevity of seeds of forest-forming species, which cosntitutes a global challenge in the sphere of retaining the biodiversity of ecosystems. I delve into the analysis of processes that shape seed stability ex situ, all the way to the molecular level, such as oxidative post-translation protein modifications and regulation of the redox status with the participation of nicotinamide adenine dinucleotide. I focus on differences between resistant (orthodox) seeds and (recalcitrant) seeds susceptible to desiccation to provide a multi-level characterisation of viability loss. I study mechanisms covering protection, repair and detoxification of seeds, shaped at the stages of maturing, drying, germination and seedling formation. I also attempt to explore new compounds, discover new interactions and biochemical processes allowing me to explain the heterogeneity of seed longevity in various tree species.
Keywords:
carbonylation, methionine sulfoxide, redox status, dehydrins, heat shock proteins, chloroplasts, photosynthesis
Publications
- Farooq M.A., Kalemba E.M., Balestrazzi A. 2024. Editorial: Underlying mechanisms transitioning seeds to seedlings. Frontiers in Plant Science 15: 1503165. https://doi.org/10.3389/fpls.2024.1503165
- Kalemba E.M., Gevaert K., Impens F., Dufour S., Czerwoniec A. 2024. The association of protein-bound methionine sulfoxide with proteomic basis for aging in beech seeds. BMC Plant Biology 24 (1): 377. https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-05085-6
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Lira J.M.S., Chmielarz P.2024. In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach. Plant Cell Tissue and Organ Culture 156(1): 24. https://link.springer.com/article/10.1007/s11240-023-02656-7
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Mendes M.M., Naskret-Barciszewska M.Z., Barciszewski J., Chmielarz P.2024. Differential morphophysiological and epigenetic responses during in vitro multiplication of Quercus robur depending on donor age and plant growth regulators. Plant Cell Tissue and Organ Culture 159(3): 62. https://doi.org/10.1007/s11240-024-02914-2
- Chmielarz P., Kotlarski S., Kalemba E.M., Martins J.P.R., Michalak M.2023. Successful in vitroshoot multiplication of Quercus robur trees aged up to 800 years. Plants 12(12): 2230. https://doi.org/10.3390/plants12122230
- Kalemba E.M., Corbineau F., Kumar S.P.J. 2023. Editorial: Molecular basis of seed longevity. Frontiers in Plant Science 14: 1138139. https://doi.org/10.3389/fpls.2023.1138139
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Mendes M.M., Chmielarz P. 2023. Calcium silicate mitigates the physiological stress induced by 6-benzylaminopurine during the in vitromultiplication of Quercus robur. Industrial Crops and Products 194: 116377. https://doi.org/10.1016/j.indcrop.2023.116377
- Kalemba E.M., Valot B., Job D., Bailly Ch., Meimoun P. 2022. Are methionine sulfoxide-containing proteins related to seed longevity? a case study of Arabidopsis thalianadry mature seeds using cyanogen bromide attack and two-dimensionaldiagonal electrophoresis. Plants 11(4): 569. https://doi.org/10.3390/plants11040569
- Martins J.P.R., Wawrzyniak M.K.,Ley-López J.M., Kalemba E.M., Mendes M.M., Chmielarz P.2022. 6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots. Plant Cell, Tissue and Organ Culture 151(1): 149-164. http://dx.doi.org/10.21203/rs.3.rs-1573274/v1
- Szuba A., Kalemba E.M., Wawrzyniak M.K., Suszka J., Chmielarz P.2022. Deterioration in the quality of recalcitrant Quercus roburseeds during six months of storage at subzero temperatures: ineffective activation of prosurvival mechanisms and evidence of freezing stress from an untargeted metabolomic study. Metabolites 12(8): 756. https://doi.org/10.3390/metabo12080756
- Wawrzyniak M.K., Kalemba E.M., Wyka T.P., Chmielarz P.2022. Changes in reserve materials deposited in cotyledons of pedunculate oak (Quercus robur) seeds during 18 months of storage. Forests 13(12): 2142. https://doi.org/10.3390/f13122142
- Alipour S., Bilska K, Stolarska E., Wojciechowska N., Kalemba E.M.2021. Nicotinamide adenine dinucleotides are associated with distinct redox control of germination in Acerseeds with contrasting physiology. PLOS One. 16(1): #e0245635. https://doi.org/10.1371/journal.pone.0245635
- Kalemba E.M., Alipour S., Wojciechowska N. 2021. NAD(P)H Drives the ascorbate–glutathione cycle and abundance of catalase in developing beech seeds differently in embryonic axes and cotyledons. Antioxidants 10(12), #2021 https://doi.org/10.3390/antiox10122021
- Kalemba E.M., Wawrzyniak M.K., Suszka J., Chmielarz P. 2021. Thermotherapy and storage temperature manipulations limit the production of reactive oxygen species in stored pedunculate oak acorns. Forests 12 (10), #1338. https://doi.org/10.3390/f12101338
- Wojciechowska N., Bagniewska-Zadworna A., Minicka J., Michalak K.M., Kalemba E.M. 2021. Localization and dynamics of the methionine sulfoxide reductases MsrB1 and MsrB2 in beech seeds. International Journal of Molecular Sciences 22(1): #402. 3390/ijms22010402
- Alipour S., Wojciechowska N., Stolarska E., Bilska K., Kalemba E.M.2020. NAD(P)-driven redox status contributes to desiccation tolerance in Acer Plant Cell Physiology 61(6): #11581167. https://doi.org/10.1093/pcp/pcaa044
- Stolarska E., Bilska K., Wojciechowska N., Bagniewska-Zadworna A., Rey P., Kalemba E.M.2020. Integration of MsrB1 and MsrB2 in the redox network during the development of orthodox and recalcitrant Acer Antioxidants (Basel) 9(12): #1250. https://doi.org/10.3390/antiox9121250
- Wawrzyniak M.K., Kalemba E.M., Ratajczak E., Chmielarz P.2020. Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus aviumand Prunus padus. PLOS One 15(6): #e0234510. https://doi.org/10.1371/journal.pone.0234510
- Wojciechowska N., Alipour S., Stolarska E., Bilska K., Rey P., Kalemba E.M.2020. Involvement of the MetO/Msr system in two Acer species that display contrasting characteristics during germination. International Journal of Molecular Sciences 21(23): #9197. https://doi.org/10.3390/ijms21239197
- Wojciechowska N., Marzec-Schmidt K., Kalemba E.M., Ludwików A., Bagniewska-Zadworna A. 2020. Seasonal senescence of leaves and roots of Populus trichocarpa - is the scenario the same or different? Tree Physiology 40(8): 987-1000. https://doi.org/10.1093/treephys/tpaa019
- Wojciechowska N., Alipour S., Stolarska E., Bilska K., Rey P., Kalemba E.M.2020. Peptide-Bound Methionine Sulfoxide (MetO) levels and MsrB2 abundance are differentially regulated during the desiccation phase in contrasted Acer seeds. Antioxidants 9(5): #391. https://doi.org/10.3390/antiox9050391
- Bilska K., Wojciechowska N., Alipour S.,Kalemba E.M. 2019. Ascorbic Acid – the little-known antioxidant in woody plants. Antioxidants 8(12): #645. https://doi.org/10.3390/antiox8120645
- Kalemba E.M., Bagniewska-Zadworna A., Suszka J., Pukacka S. 2019. Dehydration Sensitivity at the Early Seedling Establishment Stages of the European Beech (Fagus sylvatica ). Forests 10: #900. https://doi.org/10.3390/f10100900
- Kalemba E.M., Bagniewska-Zadworna A., Suszka J., Pukacka S. 2019. Dehydration sensitivity at the early seedling establishment stages of the European beech (Fagus sylvatica). Forests 10: #900. https://doi.org/10.3390/f10100900
- Kalemba E.M., Stolarska E. 2019. Regulation of gene expression of methionine sulfoxide reductases and their new putative roles in plants. International Journal of Molecular Sciences 20(6): #1309. https://doi.org/10.3390/ijms20061309
- Ratajczak E., Dietz K.J., Kalemba E.M.2019. The occurrence of peroxiredoxins and changes in redox state in Acer platanoidesand Acer pseudoplatanus during seed development. Journal of Plant Growth Regulation 38: 298-314. https://doi.org/10.1007/s00344-018-9841-8
- Bogdziewicz M., Bonal R., Espelta J.M., Kalemba E.M., Steele M.A., Zwolak R. 2018. Invasive oaks escape pre-dispersal insect seed predation and trap enemies in their seeds. Integrative Zoology 13(3): 228-237. https://doi.org/10.1111/1749-4877.12285
- Kalemba E.M., Ratajczak E.2018. The effect of a doubled glutathione level on parameters affecting the germinability of recalcitrant Acer saccharinumseeds during drying. Journal of Plant Physiology 223: 72-83. https://doi.org/10.1016/j.jplph.2018.02.010
- Wojciechowska N., Marzec-Schmidt K., Kalemba E.M., Zarzyńska-Nowak A., Jagodziński A.M., Bagniewska-Zadworna A. 2018. Autophagy counteracts instantaneous cell death during seasonal senescence of the fine roots and leaves in Populus trichocarpa. BMC Plant Biology 18(1): #260. https://doi.org/10.1186/s12870-018-1439-6
- Borek S., Kalemba E.M., Pukacka S., Pietrowska-Borek M., Stawiński S., Ratajczak L. 2017. Nitrate simultaneously enhances lipid and protein accumulation in developing yellow lupin cotyledons cultured in vitro, but not under field conditions. Journal Plant Physiology 216: 26-34. https://doi.org/10.1016/j.jplph.2017.03.021
- Hazubska-Przybył T., Kalemba E.M., Ratajczak E., Bojarczuk K. 2016. Effect of abscisic acid and an osmoticum on the maturation, starch accumulation and germination of Picea somatic embryos. Acta Physiologiae Plantarum 38: #59. http://dx.doi.org/10.1007/s11738-016-2078-x
- Kalemba E.M., Bagniewska-Zadworna A., Ratajczak E.2015. Multiple subcellular localizations of dehydrin-like proteins in the embryonic axes of common beech (Fagus sylvatica ) seeds during maturation and dry storage. Journal of Plant Growth Regulation 34: 137-149. https://doi.org/10.1007/s00344-014-9451-z
- Kalemba E.M., Litkowiec M. 2015. Functional characterization of a dehydrin protein from Fagus sylvatica seeds using experimental and in silico approaches. Plant Physiology Biochemistry 97: 246-54. https://doi.org/10.1016/j.plaphy.2015.10.011
- Kalemba E.M., Suszka J., Ratajczak E.2015. The role of oxidative stress in determining the level of viability of black poplar (Populus nigra) seeds stored at different temperatures. Functional Plant Biology 42: 630-642. https://doi.org/10.1071/FP14336
- Ratajczak E., Małecka A., Bagniewska-Zadworna A., Kalemba E.M.2015. The production, localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech (Fagus sylvatica) seeds. Journal Plant Physiology 174: 147-156. https://doi.org/10.1016/j.jplph.2014.08.021
- Ratajczak E., Kalemba E.M., Pukacka S. 2015. Age-related changes in protein metabolism of beech (Fagus sylvatica ) seeds during alleviation of dormancy and in early stage of germination. Plant Physiology and Biochemistry 94: 114-121. https://doi.org/10.1016/j.plaphy.2015.06.003
- Ratajczak E., Małecka A., Bagniewska-Zadworna A., Kalemba E.M.2015. The production, localization and spreading of reactive oxygen species contributes to the low vitality of long-term stored common beech (Fagus sylvatica ) seeds. Journal Plant Physiology 174: 147-156. https://doi.org/10.1016/j.jplph.2014.08.021
- Ratajczak E., Kalemba E.M., Pukacka S. 2015. Age-related changes in protein metabolism of beech (Fagus sylvatica) seeds during alleviation of dormancy and in early stage of germination. Plant Physiology and Biochemistry 94: 114-121. https://doi.org/10.1016/j.plaphy.2015.06.003
- Hazubska-Przybyl T., Ratajczak E.,Kalemba E.M., Bojarczuk K. 2013. Growth regulators and guaiacol peroxidase activity during the induction phase of somatic embryogenesis in Picea Dendrobiology 69: 77-86. https://dx.doi.org/10.12657/denbio.069.009
- Ratajczak E., Ströher E., Oelze M.L., Kalemba E.M., Pukacka S., Dietz K.J. 2013. The involvement of the mitochondrial peroxiredoxin Prx IIF in defining physiological differences between orthodox and recalcitrant seeds of two Acer Functional Plant Biology 40: 1005-1017. https://doi.org/10.1071/FP13002
- Kalemba E.M., Pukacka S. 2012. Association of protective proteins with dehydration and desiccation of orthodox and recalcitrant category seeds of three Acergenus species. Journal of Plant Growth Regulation 31: 351-362. https://doi.org/10.1007/s00344-011-9246-4
- Pukacka S., Ratajczak E., Kalemba E.M.2011. The protective role of selenium in recalcitrant Acer saccharinum seeds subjected to desiccation. Journal Plant Physiology 168: 220-225. https://doi.org/10.1016/j.jplph.2010.07.021
- Kalemba E.M., Janowiak F, Pukacka S. 2009. Desiccation tolerance acquisition in developing beech (Fagus sylvatica ) seeds: the contribution of dehydrin-like protein. Trees 23: 305-315. https://doi.org/10.1007/s00468-008-0278-8
- Pukacka S., Ratajczak E., Kalemba E.M.2009. Non-reducing sugar levels in beech (Fagus sylvatica) seeds as related to withstanding desiccation and storage. Journal Plant Physiology 166: 1381-1390. https://doi.org/10.1016/j.jplph.2009.02.013
- Kalemba E.M., Pukacka S. 2008. Changes in late embryogenesis abundant proteins and small heat shock protein during storage of beech (Fagus sylvatica ) seeds. Environmental and Experimental Botany 63: 274-280. https://doi.org/10.1016/j.envexpbot.2007.12.011
- Kalemba E., Pukacka S. 2007. Possible roles of LEA proteins and sHSPs in seed protection, a short review. Biological Lettres 44: 1-8.
Projects
-
Significance of chlorophyll, chloroplasts, redox balance driven by nicotinamide adenine dinucleotides and metabolites in desiccation tolerance and longevity in woody plant chloroembryos. (Original title: Znaczenie chlorofilu, chloroplastów, równowagi redoks zależnej od dinukleotydów nikotynoamidoadeninowych i metabolitów w tolerancji na desykację i w żywotności zielonych nasion roślin drzewiastych). Project funded by the National Science Centre (OPUS, 2023/49/B/NZ9/00828). Project duration: 2024–2028. Principal Investigator: Kalemba E.M.
-
Identification and quantitative analysis of primary and secondary metabolites in germinating Norway maple and sycamore maple seeds. (Original title: Identyfikacja oraz analiza ilościowa metabolitów pierwotnych i wtórnych w kiełkujących nasionach klonu zwyczajnego i klonu jawora). Project funded by the Internal Research Fund of the Institute of Dendrology, Polish Academy of Sciences (FBW_3, DEC-2022/03/ZB/FBW/00004). Project duration: 2022–2023. Principal Investigator: Kalemba E.M.
-
The role of proteins containing oxidized methionine and the methionine sulfoxide reductase system in regulating key developmental stages in seed physiology. (Original title: Udział białek zawierających utlenioną metioninę oraz systemu reduktaz sulfotlenku metioniny w regulacji podstawowych etapów rozwojowych w fizjologii nasion). Project funded by the National Science Centre (OPUS, 2015/18/E/NZ9/00729). Project duration: 2016–2020. Principal Investigator: Kalemba E.M.
-
Carbonylation of proteins in seeds of European beech (Fagus sylvatica) during long-term storage. (Original title: Karbonylacja białek w nasionach buka zwyczajnego (Fagus sylvatica L.) podczas długoterminowego przechowywania). Project funded by the Ministry of Science and Higher Education (2980/B/P01/2010/39). Project duration: 2010–2011. Principal Investigator: Kalemba E.M.
-
Functional analysis of dehydrines and LEA-like proteins in seeds of selected species of deciduous trees. (Original title: Analiza funkcjonalna dehydryn i białek podobnych do LEA (LEA-like) w nasionach wybranych gatunków drzew liściastych). Project funded by the Ministry of Science and Higher Education (1365/B/P01/2008/35). Project duration: 2008–2011. Principal Investigator: Kalemba E.M.
Popular Science Articles
[Articles in Polish]
- Kalemba E.M.2024. Biopriming, czyli jak można poprawić jakość nasion używanych jako materiał siewny w sposób przyjazny dla środowiska. Wszechświat 125(10-12): 250-255.
- Kalemba E.2023. Kto miód łyka, jak koń bryka. Kórniczanin 22: 15.
- Kalemba E.M.2022. Wszystkie oblicza witaminy C. Wiedza i Życie 3: 48-52.
- Kalemba E.M.2021. Zielona armia. Wiedza i Życie 9: 10-15.
- Kalemba E.2020. Jak rozmnaża się klon zwyczajny? Kórniczanin 22: #13.
- Kalemba E.M. 2020. Cenne nasiona. Wiedza i Życie 11: 62-65
- Kijowska-Oberc J., Kalemba E.M., Ratajczak E. Wszystko zaczyna się od nasion. Głos Lasu 2: 24-26.
- Kalemba E.M.2019. Ożeż ty orzeszku! czyli o dobrodziejstwie drzew dla człowieka i zwierząt. Przyroda Polska 11(978): 8-9.
- Wawrzyniak M.K., Ratajczak E.,Kalemba E., Chmielarz P.2019. Las zaczyna się od nasion [The forest starts with the seeds]. Academia – Magazyn Polskiej Akademii Nauk 3-4: 59-60.
Books and Book Chapters
- Kalemba E.M.2024. Factors determining seed viability and successful seed germination in a changing world. In: Tomaszewski D., Jagodziński A.M. (eds.). Research and Practice in Forest Ecology. International Scientific Conference for Young Scientists. Kórnik, 8-12 May 2024. Book of abstracts. Bogucki Wydawnictwo Naukowe, Poznań. Pp. 69-75.
[Dr. Habil., Prof. ID PAS]
Position: Associate Professor
Building A, Room 2
Phone: +48 61 817 00 33 ext. 146
e-mail:
Represented disciplines:
Biological sciences
Forestry sciences
Other profiles:
ORCID: https://orcid.org/0000-0002-0092-0339
Scopus: https://www.scopus.com/authid/detail.uri?authorId=23492583500
ResearchGate: https://www.researchgate.net/profile/Ewa-Kalemba









