Research focus:
My research focuses on the anatomical and physiological responses of woody plants under in vitro conditions, with particular emphasis on improving their morphophysiological quality and understanding the mechanisms of physiological stress. My primary goal is to optimize micropropagation protocols for tree species, especially those that are recalcitrant to conventional propagation methods, and to elucidate how plants adapt to oxidative and hypoxic stress during controlled cultivation.
I investigate the effects of various plant growth regulators, including exogenous cytokinins and auxins, as well as other beneficial elements, such as silicon and activated charcoal, on the physiological quality of plantlets and the performance of their photosynthetic apparatus. Special attention is given to oxidative mechanisms and hormonal balance, and how they influence tissue development in leaves and roots, as well as the accumulation of starch and photosynthetic pigments. I adopt an integrative approach combining anatomical, biochemical, and physiological analyses to comprehensively assess plant quality in vitro. My research supports advancements in forest resource conservation, plant biotechnology, and the clonal propagation of valuable tree genotypes such as Quercus robur, particularly in the context of biodiversity preservation and improved acclimatization success.
Keywords:
chlorophyll a fluorescence, in vitro culture, plant anatomy, plant growth regulator, plant physiology, physiological disorders, woody plant species
Publications
- Martins J.P.R., Wawrzyniak M.K., Ley-López J.M., Mendes M.M., Mokhtari A.M., Chmielarz P. 2025. Regulation of the cytokinin metabolic spectrum and morphogenesis in Quercus robur (L.) in vitro by exogenous cytokinins and activated charcoal. Industrial Crops and Products 233: 121362. https://doi.org/10.1016/j.indcrop.2025.121362
- Martins J.P.R., Mokhtari A.M., Wawrzyniak M.K. 2025. Cytokinins combined with activated charcoal do not impair in vitro rooting in Quercus robur L.: insights from morphophysiological and hormonal analyses. BMC Plant Biology 25(1): 1005. https://link.springer.com/article/10.1186/s12870-025-07064-x
- Chmielarz P., Sánchez C., Martins J.P.R., Ley-López J.M., Covelo P., Cernadas M.J., Aldrey A., Rico S., Vielba J.M., Christie B., Vidal N. 2025. Multiplication of axillary shoots of adult Quercus robur L. trees in RITA® bioreactors. Forests 16(8): 1285. https://doi.org/10.3390/f16081285
- Mendes M.M., Miranda J.F., Neves L.B., Martins J.P.R., Rocha A.C.P., Cassol D., Gontijo A.B.P.L., Falqueto A.R. 2025. Boron excess in Alternanthera tenella Colla under in vitro conditions: tolerance and potential for supplementation in plants. Plant Cell, Tissue and Organ Culture 163: 117. https://link.springer.com/article/10.1007/s11240-025-03313-x
- Mendes M.M., Ribeiro I.F., Santos V.F.D., Pires F.R., Fernandes A.A., Menezes L.F.T.D., Bonomo R., Cassol D., Martins J.P.R., Falqueto A.R. 2025. Green manure can be an auxiliary factor against dynamic photoinhibition in Dalbergia ecastophyllum (L.) young trees in areas impacted by mining. Photosynthetica 63(2): 129-139. DOI: 10.32615/ps.2025.015
- Rocha A.C.P., Miranda J.F., Das Neves L.B., Mendes M.M., Amorim A.S., Morais G.S., Martins J.P.R., Da Silva M.B., Gontijo A.B.P.L., Falqueto A.R. 2025. Effect of strobilurin fungicide on the initial growth of common bean plants. Photosynthetica 63(2): 116-128. DOI: 10.32615/ps.2025.017
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Lira J.M.S., Chmielarz P. 2024. In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach. Plant Cell Tissue and Organ Culture 156(1): 24. https://link.springer.com/article/10.1007/s11240-023-02656-7
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Mendes M.M., Naskret-Barciszewska M.Z., Barciszewski J., Chmielarz P. 2024. Differential morphophysiological and epigenetic responses during in vitro multiplication of Quercus robur depending on donor age and plant growth regulators. Plant Cell Tissue and Organ Culture 159(3): 62. https://doi.org/10.1007/s11240-024-02914-2
- Wasileńczyk U., Wawrzyniak M.K., Martins J.P.R., Kosek P., Chmielarz P. 2024. Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying. Plant Methods 20(1): 53. https://doi.org/10.1186/s13007-024-01161-y
- Chmielarz P., Kotlarski S., Kalemba E.M., Martins J.P.R., Michalak M.2023. Successful in vitro shoot multiplication of Quercus robur L. trees aged up to 800 years. Plants 12(12): 2230. https://doi.org/10.3390/plants12122230
- Cipriano R., Martins J.P.R., Conde L.T., Silva M.M., Silva D.M., Gontijo A.B.P.L., Falqueto A.R. 2023. Anatomical and physiological responses of Aechmea blanchetiana (Bromeliaceae) induced by silicon and sodium chloride stress during in vitro culture. PeerJ 11: e14624. https://doi.org/10.7717/peerj.14624
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Mendes M.M., Chmielarz P. 2023. Calcium silicate mitigates the physiological stress induced by 6-benzylaminopurine during the in vitro multiplication of Quercus robur. Industrial Crops and Products 194: 116377. https://doi.org/10.1016/j.indcrop.2023.116377
- Pinheiro C.L., Zampirollo J.B., Mendes M.M., dos Santos V.F., Martins J.P.R., Silva D.M. Tognella M.M.P., Cassol D., Falqueto A.R. 2023. Exposition of three Cattleya species (Orchidaceae) to full sunlight: effect on their physiological plasticity and response to changes in light conditions. Ornamental Horticulture 29(1):57-67. http://dx.doi.org/10.1590/2447-536x.v29i1.2527
- Wawrzyniak M.K., Ley-López J.M., Martins J.P.R., Chmielarz P. 2023. Limiting temperatures of embryo growth and seed germination of Fraxinus excelsior L. (Oleaceae): a threshold model approach. Annals of Forest Science 80(1): 38. https://doi.org/10.1186/s13595-023-01205-8
- Martins J.P.R., Braga P.C.S., Rossini F.P., Conde L.T., Cipriano R., Falqueto A.R., Gontijo A.B.P.L. 2022. In vitro clonal propagation of Billbergia zebrina (Bromeliaceae) and analysis of anatomy and physiology of regenerated plantlets. Israel Journal of Plant Sciences 69(3-4): 129-139. https://doi.org/10.1163/22238980-bja10054
- Martins J.P.R., Rodrigues L.C.A., Braga P.C.S., Falqueto A.R., Gontijo A.B.P. 2022. Impacts of copper on photosynthetic pigments and anatomy of Alcantarea imperialis (Bromeliaceae) under in vitro conditions. Biotechnology Agronomie Societe Environment 26(2): 78-87. https://doi.org/10.25518/1780-4507.19633
- Martins J.P.R., Wawrzyniak M.K., Ley-López J.M., Kalemba E.M., Mendes M.M., Chmielarz P. 2022. 6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots. Plant Cell, Tissue and Organ Culture 151(1): 149-164. http://dx.doi.org/10.21203/rs.3.rs-1573274/v1
Projects
- Slow-growing storage of 800-year-old Quercus robur clones: impacts of in vitro conditions on regenerative capacity, regrowth, and physiological status. (Original title: Przechowywanie 800-letnich klonów Quercus robur w warunkach ograniczających wzrost: wpływ hodowli in vitro na zdolność regeneracyjną, odrastanie i stan fizjologiczny). Projekt finansowany przez Narodowe Centrum Nauki (MINIATURA, DEC-2024/08/X/NZ9/00281). Okres realizacji 2024-2025. Kierownik projektu: Martins J.P.R.
- Regenerative capacity, morphophysiology, and hormonal balance of Quercus robur shoots during in vitro propagation: synthetic cytokinins vs. activated charcoal. Project funded by the Internal Research Fund of the Institute of Dendrology, Polish Academy of Sciences (FBW_4, DEC-2024/04/ZB/FBW/01). Project duration: 2024–2025. Principal Investigator: Martins J.P.R.
Popular Science Articles
[Articles in Polish]
- Martins J. P. R., Wawrzyniak M. K. 2024. BIOróżnorodność w słoiku. Kultury in vitro jako narzędzie ochrony gatunków roślin. Salamandra 1: 24-26.
Books and Book Chapters
- Hazubska-Przybył T., Chmielarz P., Wawrzyniak M.K., Martins J.P.R., Kijowska-Oberc J., Fuchs H., Ratajczak E., Szuba A., Karliński L., Forysiak K., Sęktas J. 2024. In vitro cultures used in biology of forest tree species – New research opportunities. W: Sota W., Werbrouck S. In Vitro Culture of Woody Crops: Problem Solving by New Approaches. Book of Proceedings. The 2nd Conference of Cost Action CA21157 CopyTree, 22-24 April, Jurmala, Łotwa, SS.90-101.
- Chmielarz P., Suszka J., Wawrzyniak M.K., Kotlarski S., Plitta-Michalak B.P., Michalak M., Martins J.P.R., Ley-López J.M. 2024. Can we conserve exceptional forest tree species ex situ in the face of global climate change? In: Tomaszewski D., Jagodziński A.M. (eds.). Research and Practice in Forest Ecology. International Scientific Conference for Young Scientists. Kórnik, 8-12 May 2024. Book of abstracts. Bogucki Wydawnictwo Naukowe, Poznań. Pp. 59-67.
[PhD]
Position: Assistant Professor
Building A, Room 11C
Phone: +48 61 817 00 33 ext. 197
e-mail:
Represented disciplines:
Biological sciences
Forestry
Other profiles:
ORCID: https://orcid.org/0000-0003-0554-6793
Scopus: https://www.scopus.com/authid/detail.uri?authorId=54950623000
ResearchGate: https://www.researchgate.net/profile/Joao-Paulo-Martins-5
Ludzie Nauki: https://ludzie.nauka.gov.pl/ln/profiles/joaopaulo.rodriguesmartins.gLv3ggQd6rH









