Research focus:
I am a scholar specialising in the biology of forest tree seeds and in the retention of plant genetic resources. In my research, I focus on the understanding of the effect of environmental factors on seed dormancy and germination. A crucial part of my work is the issue of seed storage and its effect on seed viability and the further growth of seedlings. I place special emphasis on the seeds that are difficult to store (recalcitrant seeds). Their uniqueness is related to their resistance to water loss, which makes their long-term storage impossible. In order to effectively secure the genetic resources of such plant species, I extend my studies with cryopreservation techniques used with seed materials and in vitro plant breeding. In my work, I hope to get insight into seed biology and develop effective methods for seed storage. It is vital to effectively secure plant genetic resources in order to maintain biodiversity and successfully cultivate forest trees.
Keywords:
seed storage, seeds, in vitro, cryopreservation, dormancy, germination
Publications
- Hazubska-Przybył T., Obarska A., Konecka A., Kijowska-Oberc J., Wawrzyniak M.K., Piotrowska-Niczyporuk A., Staszak A.M., Ratajczak E. 2024. Modulating ascorbic acid levels to optimize somatic embryogenesis in Picea abies (L.) H. Karst. Insights into oxidative stress and endogenous phytohormones regulation. Frontiers in Plant Science 15: 1372764. https://doi.org/10.3389/fpls.2024.1372764
- Hazubska-Przybył T., Wawrzyniak M.K., Obarska A., Salaj T. 2024. Cryopreservation of Abies alba x A. numidica and Pinus nigra embryogenic tissues by stepwise dehydration method. Plant Methods 20(1): 10. https://plantmethods.biomedcentral.com/articles/10.1186/s13007-023-01131-w
- Jaganathan G.K., Canelo T., Phartyal S.S., Li J.J., Kang H., Chmielarz P., Wawrzyniak M.K., Tewari A., Shah S., Liu B.L., Sánchez J.A., Berry K. 2024. The reproductive biology of Fagaceae acorns in the current and future climate. Flora 315: 152504. https://doi.org/10.1016/j.flora.2024.152504
- Kijowska-Oberc J., Wawrzyniak M.K., Ciszewska L., Ratajczak E. 2024. Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress. PeerJ 12: e16697. https://doi.org/10.7717/peerj.16697
- Ley-López J.M., Alvarado C.M., Valerio E.R., Wawrzyniak M.K., Chmielarz P. 2024. Phenological patterns from two sympatric subspecies of the palm Geonoma cuneata (H. Wendl. ex Spruce) and their gall inductor Contarinia geonomae (Gagné). Dendrobiology 91: 70-84. https://doi.org/10.12657/denbio.091.006
- Maleki K., Chmielarz P., Wawrzyniak M.K., Maleki K., Maleki A., Soltani E. 2024. Seed regeneration in Taxus baccata: Unveiling Ecological restrictions and paving the way for future studies. Ecology and Evolution 14(11): 70534. https://doi.org/10.1002/ece3.70534
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Lira J.M.S., Chmielarz P. 2024. In vitro rooting of Quercus robur, activated charcoal vs. exogenous auxin: a morphophysiological approach. Plant Cell Tissue and Organ Culture 156(1): 24. https://link.springer.com/article/10.1007/s11240-023-02656-7
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Mendes M.M., Naskret-Barciszewska M.Z., Barciszewski J., Chmielarz P. 2024. Differential morphophysiological and epigenetic responses during in vitro multiplication of Quercus robur depending on donor age and plant growth regulators. Plant Cell Tissue and Organ Culture 159(3): 62. https://doi.org/10.1007/s11240-024-02914-2
- Wasileńczyk U., Wawrzyniak M.K., Martins J.P.R., Kosek P., Chmielarz P. 2024. Cryopreservation of sessile oak (Quercus petraea (Matt.) Liebl.) plumules using aluminium cryo-plates: influence of cryoprotection and drying. Plant Methods 20(1): 53. https://doi.org/10.1186/s13007-024-01161-y
- Martins J.P.R., Wawrzyniak M.K., Kalemba E.M., Ley-López J.M., Mendes M.M., Chmielarz P. 2023. Calcium silicate mitigates the physiological stress induced by 6-benzylaminopurine during the in vitro multiplication of Quercus robur. Industrial Crops and Products 194: 116377. https://doi.org/10.1016/j.indcrop.2023.116377
- Wawrzyniak M.K., Ley-López J.M., Martins J.P.R., Chmielarz P. 2023. Limiting temperatures of embryo growth and seed germination of Fraxinus excelsior L. (Oleaceae): a threshold model approach. Annals of Forest Science 80(1): 38. https://doi.org/10.1186/s13595-023-01205-8
- Chmielarz P., Suszka J., Wawrzyniak M.K. 2022. Desiccation does not increase frost resistance of pedunculate oak (Quercus robur L.) seeds. Annals of Forest Science 79: 3. https://doi.org/10.1186/s13595-022-01121-3
- Hazubska-Przybył T., Wawrzyniak M.K., Kijowska-Oberc J., Staszak A.M., Ratajczak E. 2022. Somatic embryogenesis of Norway Spruce and Scots Pine: Possibility of application in modern forestry. Forests 13(2): 155. https://doi.org/10.3390/f13020155
- Kijowska-Oberc J., Wawrzyniak M.K., Staszak A.M., Ratajczak E. 2022. Exogenous seed treatment with proline and its consequences to Norway spruce (Picea abies (L.) H. Karst) seedling establishment. Dendrobiology 87: 149-162. http://dx.doi.org/10.12657/denbio.087.011
- Martins J.P.R., Wawrzyniak M.K., Ley-López J.M., Kalemba E.M., Mendes M.M., Chmielarz P. 2022. 6-Benzylaminopurine and kinetin modulations during in vitro propagation of Quercus robur (L.): an assessment of anatomical, biochemical, and physiological profiling of shoots. Plant Cell, Tissue and Organ Culture 151(1): 149-164. http://dx.doi.org/10.21203/rs.3.rs-1573274/v1
- Szuba A., Kalemba E.M., Wawrzyniak M.K., Suszka J., Chmielarz P. 2022. Deterioration in the quality of recalcitrant Quercus robur seeds during six months of storage at subzero temperatures: ineffective activation of prosurvival mechanisms and evidence of freezing stress from an untargeted metabolomic study. Metabolites 12(8): 756. https://doi.org/10.3390/metabo12080756
- Wawrzyniak M.K., Kalemba E.M., Wyka T.P., Chmielarz P. 2022. Changes in reserve materials deposited in cotyledons of pedunculate oak (Quercus robur L.) seeds during 18 months of storage. Forests 13(12): 2142. https://doi.org/10.3390/f13122142
- Wawrzyniak M.K., Ley-Lopez J.M., Kijowska-Oberc J., Chmielarz P., Ratajczak E. 2022. Effects of spermidine on germination of Salix spp. after storage under controlled conditions. Dendrobiology 87: 137-148. http://dx.doi.org/10.12657/denbio.087.010
- Kalemba E.M., Wawrzyniak M.K., Suszka J., Chmielarz P. 2021. Thermotherapy and storage temperature manipulations limit the production of reactive oxygen species in stored pedunculate oak acorns. Forests 12 (10), #1338. https://doi.org/10.3390/f12101338
- Kijowska-Oberc J., Staszak A. M., Wawrzyniak M. K., Ratajczak E. 2021. Changes in proline levels during seed development of orthodox and recalcitrant seeds of genus Acer in a climate change scenario. Forests, 11(12): #1362. https://doi.org/10.3390/f11121362
- Suszka J., Bujarska-Borkowska B., Tylkowski T., Wawrzyniak M.K. 2021. Effects of temperature, moisture content and storage on dormancy release and germination of European hornbeam (Carpinus betulus L.) seeds. Forestry 2021: 1–9. https://doi.org/10.1093/forestry/cpab053
- Kijowska-Oberc J., Staszak A.M., Wawrzyniak M.K., Ratajczak E. 2020. Changes in proline levels during seed development of orthodox and recalcitrant seeds of Genus Acerin a climate change scenario. Forests 11: #1362. https://doi.org/10.3390/f11121362
- Wawrzyniak M.K., Jasińska A.K., Chmielarz P., Kozlowski G. 2020. Desiccation, dormancy, and storage of Pterocarya fraxinifolia (Juglandaceae) seeds: application in Hyrcanian and Colchian conservation. Canadian Journal of Forest Research 50: 24-31. https://doi.org/10.1139/cjfr-2018-0519
- Wawrzyniak M.K., Kalemba E.M., Ratajczak E., Chmielarz P. 2020. Oxidation processes related to seed storage and seedling growth of Malus sylvestris, Prunus avium and Prunus padus. PLOS One 15(6): #e0234510. https://doi.org/10.1371/journal.pone.0234510
- Wawrzyniak M.K., Michalak M., Chmielarz P. 2020. Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants. Annals of Forest Science 77: 1-20. https://doi.org/10.1007/s13595-020-00963-z
- Wawrzyniak M.K., Ratajczak E., Kalemba E., Chmielarz P. 2019. Las zaczyna się od nasion [The forest starts with the seeds]. Academia 3-4: 59-60.
- Hazubska-Przybył T., Wawrzyniak M. 2017. Stimulation of embryo growth and development in Picea spp. by polyethylene glycol. Dendrobiology 78: 168-178. http://dx.doi.org/10.12657/denbio.078.016
- Wawrzyniak M.K., Szwengiel A., Stelmach-Mardach M., Hołubowicz R. 2016. Effects of Chlorocholine Chloride (CCC) on plant height and inulin content in Jerusalem artichoke (Helianthus tuberosus L.). Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Horticulture, 73(2): 210-216. http://dx.doi.org/10.15835/buasvmcn-hort:11957
- Hazubska-Przybył T., Wawrzyniak M., Obarska A., Bojarczuk K. 2015. Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce. Acta Physiologiae Plantarum 37(1): 1-9. https://doi.org/10.1007/s11738-014-1735-1
Projects
- Impact of the genotype of individual Quercus robur trees with desired functional traits on the production of their seedlings through micropropagation in in vitro cultures. (Original title: Wpływ genotypu indywidualnych drzew Quercus robur o pożądanych cechach użytkowych na uzyskanie z nich sadzonek metodą mikrorozmnażania w kulturach in vitro). Project funded by the General Directorate of State Forests (EZ.271.3.1.2024). Project duration: 2024–2029. Principal Investigator: Wawrzyniak M.K.
- The effect of glutathione and spermidine during osmopriming on the germination of Scots pine (Pinus sylvestris) seeds. (Original title: Wpływ glutationu i spermidyny podczas osmoprimingu na kiełkowanie nasion sosny zwyczajnej (Pinus sylvestris L.)). Project funded by the Internal Research Fund of the Institute of Dendrology, Polish Academy of Sciences (FBW_4, DEC-2024/04/ZB/FBW/07). Project duration: 2024–2025. Principal Investigator: Wawrzyniak M.K.
- Factors modulating rooting of pedunculate oak (Quercus robur) under in vitro conditions. (Original title: Czynniki modulujące ukorzenianie się dębu szypułkowego w warunkach in vitro). Project funded by the Internal Research Fund of the Institute of Dendrology, Polish Academy of Sciences (FBW_3, DEC-2022/03/ZB/FBW/00007). Project duration: 2022–2023. Principal Investigator: Wawrzyniak M.K.
- Germination control of Norway spruce (Picea abies (L.) H. Karst) seeds in current and future climate. (Original title: Kontrola kiełkowania nasion świerka pospolitego (Picea abies (L.) H. Karst) w obecnym i przyszłym klimacie). Project funded by the National Science Centre (MINIATURA, 2021/05/X/NZ8/00537). Project duration: 2021–2022. Person carrying out research activity: Wawrzyniak M.K.
- Seeds in a changing climate: how environmental factors regulate dormancy and germination in European ash (Fraxinus excelsior). (Original title: Nasiona w zmieniającym się klimacie: jak czynniki środowiskowe regulują spoczynek i kiełkowanie nasion jesionu wyniosłego (Fraxinus excelsior L.)). Project funded by the Internal Research Fund of the Institute of Dendrology, Polish Academy of Sciences (FBW_2, DEC-2022/02/ZB/FBW/00005). Project duration: 2022. Principal Investigator: Wawrzyniak M.K.
- Down on the wind - preserving genetic resources of short-lived seeds from the genus Salix. (Original title: Puch na wietrze - zachowanie zasobów genowych krótkowiecznych nasion z rodzaju Salix spp.). Project funded by the Internal Research Fund of the Institute of Dendrology, Polish Academy of Sciences (FBW_1, DEC-2021/01/ZB/FBW/00004). Project duration: 2021. Principal Investigator: Wawrzyniak M.K.
Popular Science Articles
[Articles in Polish]
- Martins J. P. R., Wawrzyniak M. K. 2024. BIOróżnorodność w słoiku. Kultury in vitro jako narzędzie ochrony gatunków roślin. Salamandra 1: 24-26.
- Wawrzyniak M.K. 2024. Czekając na sygnał. Kórniczanin 10: 8.
- Wawrzyniak M. 2023. O jesionie słów kilka. Las Polski 1: 18-19.
- Chmielarz P., Wawrzyniak M., Suszka J., Młynarczyk A. 2021. Karpacki Bank Genów – w ochronie beskidzkiego świerka. Las Polski 9/2021: 22-24.
- Suszka J., Chmielarz P., Wawrzyniak M. 2021. Skąd się bierze las? Kórniczanin 16/2021: 12.
- Wawrzyniak M. 2021. Kwietne aleje owocowe. Kórniczanin 8: 12.
- Wawrzyniak M. 2021. Nasiona na straży przyszłości. Salamandra. Magazyn Przyrodniczy 2/2021.
- Wawrzyniak M.K., Chmielarz P. 2020. Ochrona leśnych zasobów genowych w dobie zmian klimatycznych. Chrońmy Przyrodę Ojczystą 76(3): 38-43.
- Wawrzyniak M.K. 2019. Nasiona-skrzynki pełne skarbów. Kórniczanin 19: 16-17.
Books and Book Chapters
- Chmielarz P., Suszka J., Wawrzyniak M.K., Kotlarski S., Plitta-Michalak B.P., Michalak M., Martins J.P.R., Ley-López J.M. 2024. Can we conserve exceptional forest tree species ex situ in the face of global climate change? In: Tomaszewski D., Jagodziński A.M. (eds.). Research and Practice in Forest Ecology. International Scientific Conference for Young Scientists. Kórnik, 8-12 May 2024. Book of abstracts. Bogucki Wydawnictwo Naukowe, Poznań. Pp. 59-67.
- Hazubska-Przybył T., Chmielarz P., Wawrzyniak M.K., Martins J.P.R., Kijowska-Oberc J., Fuchs H., Ratajczak E., Szuba A., Karliński L., Forysiak K., Sęktas J. 2024. In vitro cultures used in biology of forest tree species – New research opportunities. W: Sota W., Werbrouck S. In Vitro Culture of Woody Crops: Problem Solving by New Approaches. Book of Proceedings. The 2nd Conference of Cost Action CA21157 CopyTree, 22-24 April, Jurmala, Łotwa, SS.90-101.
[Dr. Eng.]
Position: Assistant Professor
Building A, Room 11C
Phone: +48 61 817 00 33 ext. 197
e-mail:
Represented disciplines:
Biological sciences
Forestry sciences
Other profiles:
ORCID: https://orcid.org/0000-0002-4297-5741
Scopus: https://www.scopus.com/authid/detail.uri?authorId=57212931068
ResearchGate: https://www.researchgate.net/profile/Mikolaj-Wawrzyniak
Ludzie Nauki: https://ludzie.nauka.gov.pl/ln/profiles/miko%C5%82aj.wawrzyniak.AhrQQ8jf8Jy









